Baculovirus and Insect Cell Expression Protocols by David W. Murhammer

Baculovirus and Insect Cell Expression Protocols



Download Baculovirus and Insect Cell Expression Protocols




Baculovirus and Insect Cell Expression Protocols David W. Murhammer
Language: English
Page: 446
Format: pdf
ISBN: 1588295370, 9781588295378
Publisher: Humana Press

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

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